| Chemical Inventory |  | Sample Tracking |  | E-Laboratory Notebooks |  | MSDS Management |  | Chemical Drawing |  | Molecular Modeling Drawing |

SPECTRAL PROPERTIES OF THE PHTHALOCYANINES AND THEIR PHOTOTOXICITY Hana Kolarova, Roman Kubínek, Miroslav Strnad Centre of Molecular Biology and Medicine, Palacký University Olomouc, Department of Biophysics, Faculty of Medicine, Palacký University, Olomouc, Czech Republic E-mail: KOL@risc.upol.cz, KUBIN@risc.upol.cz Introduction In photodynamic therapy (PDT), a sensitizer, light, and oxygen are used to cause photochemically induced cell´s death. The mechanism of phototoxicity involves generation of singlet oxygen and other free radicals when the lightexcited senzitizer loses or accepts an electron. Efficiency of PDT is affected by factors included: absorption spectrum of the photosensitizer wavelenght of the activation light depth of the light penetration in the tissue biological tissue answer on singlet oxygen There are four properties that would make a sensitizer optimal for PDT. selective retention in tumorous tissue fast removing from healthy tissue, first of all from skin and eyes possibility of the activation by longer and depthly penetrating wavelenght featureless toxicity Types of photosensibilisers Phthalocyanines, chlorines and 5-aminolevulinic acid present a new generation of substances for photodynamic treatment of tumors. Protoporphyrin IX production after systemic 5-aminolevulinic acid administration is very tissue specific. Phthalocyanines can be chelated with a variety of metals, chiefly aluminium and zinc, and these diamagnetic metals enhance the phototoxicity. Less sulfonated compounds, which are more lipophilic, exhibit the best membrane-penetrating properties and are the most active. To find optimal therapeutic doses for i. v. or local application, it was necessary to test: the maximum non-toxic concentration minimum phototoxic level relate to laser irradiation in different model systems. Standard testing system for definition of the in vitro cytotoxicity and phototoxicity: splenocytes obtained from rabbit spleen fragments B16 pigmented melanoma