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DIFFERENT SENSITIVITY OF CELLS FROM TUMOR-BEARING ORGANISMS TO CONTINUOUS-WAVE AND PULSED LASER RADIATION (l =632.8 nm) EVALUATED BY CHEMILUMINESCENCE TEST T.I. Karua, T.P.Ryabykhb, S.N.Antonovc, V.S. Letokhovd aLaser Technology Research Center, Russian Academy of Sciences, 142092 Troitsk, Moscow Region; bN. Blokhin Cancer Research Center of Academy of Medical Sciences, Moscow; cInstitute of Radio Engineering and Electronics, Russian Academy of Sciences, Fryazino, Moscow Region; dInstitute of Spectroscopy, Russian Academy of Sciences, Troitsk, Moscow Region, Russian Federation. Corresponding author Dr. Tiina Karu <karu@lls.isan.troitsk.ru>
ABSTRACT Chemiluminescence test results were used to evaluate the sensitivity of human blood (healthy persons and colon cancer patients) and murine splenocytes (separated from intact animals and from mice with transplanted leukemia EL-4) to continuous-wave (CW) and pulsed He-Ne laser light (632.8 nm, 5x103 J/m2, 1-10 Hz, duty cycle 50 and 94%). It was demonstrated that CW radiation has in our experimental conditions practically no effect on the luminol-amplified chemiluminescence of four models under study. The pulsed radiation had a week inhibiting effect on the samples from healthy organisms but inhibited markedly the chemiluminescence of samples from tumor-bearing organisms. The effect depended on duration of dark period between pulses. A transient local heating mechanism was proposed to explain the inhibition of activity of NADPH-oxidase.
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